FastQCFastQC Report
Wed 12 Oct 2016
SRR1976948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1976948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTG4450.041.29694245
GCGTCGG23200.013.201967245
GAGCGTG7603.5144467E-412.896237245
CGGAAGG22450.012.551572245
GTCGTGG11156.324296E-510.987825245
GGAAAGG34650.09.900142245
TCTCGGG29203.6379788E-129.650095245
AGATCTG15301.4834113E-59.608961245
AGAGCGG22051.8228093E-89.445544245
CGGGTAG16553.747994E-58.883209245
GTGGGCG19550.00214683966.893385245
CGTGTGG3700.0096428286.621861240-244
CGGTGGG22800.00150899256.4481187245
CCCACCC22450.009094486.002926245
GGGGGTG33659.981489E-55.825343245
CGGGGGG114900.05.651223245
AAGGGGG45551.4725229E-65.648297245
GGGTGGG28400.00281695565.608047245
GTCGGGG26400.0077485055.5688295245
GGTGGGG36052.681071E-45.437526245
Produced by FastQC (version 0.11.2)