FastQCFastQC Report
Wed 12 Oct 2016
SRR1976948_2.qc.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1976948_2.qc.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885734
Sequences flagged as poor quality0
Sequence length25-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG6600.037.632378245
AGATCGG19400.027.52595245
TACGCGC2500.001490450124.1769262
GGGCGCG14300.00651380237.8159556245
CCCCACC27402.2736596E-46.345313245
GCCCCCC58251.2594137E-75.3299074245
GGGAAGG32850.0023001165.2925897245
Produced by FastQC (version 0.11.2)